THE USE OF A NON-IONIC SURFACTANT (P188) TO SAVE CHONDROCYTES
FROM NECROSIS FOLLOWING IMPACT LOADING OF CHONDRAL EXPLANT
Journal of Orthopaedic
Research, 22:1135-1142,2004
Abstract:
While current injury criteria for the automotive
industry are based on bone fracture,
the majority of knee injuries suffered during automobile collisions each
year do not involve fracture of bone.
Furthermore, clinical studies often document early joint pain and the
development of a chronic disease, such as osteoarthritis, in traumatized joints
without fracture of bone. Various
studies from the literature suggest this chronic disease can be initiated by
the death of cells in articular cartilage due to mechanical trauma, but the
literature is yet unclear on the pathway of cell death. Earlier studies by this laboratory, using
viability stains, indicate that mechanical loads applied to chondral explants
can damage the cell membranes of chondrocytes.
In the current investigation use of a non-ionic surfactant (P188), was
explored as a potential tool for early intervention to repair damaged cell
membranes in cartilage after blunt trauma.
Adult bovine chondral explants were loaded with a peak pressure of 30
MPa in unconfined compression. The
addition of a surfactant, P188, to the culture media immediately post-impact
reduced the percentage of cell death in the explants by approximately 50% at
both 1 and 24 hours. The current study
confirmed earlier data with other cell lines that show the ability of P188 to
repair the integrity of damaged cell membranes after mechanical trauma. With the capability of P188 to repair cell
membranes and prevent early necrotic death, its role in prevention of a
post-traumatic osteoarthrosis should be explored in studies using an in vivo animal model.
Orthopaedic Biomechanics Laboratories,
College
of Osteopathic Medicine,
Michigan
State University,
East Lansing,
Michigan 48824
Please address correspondence to:
Roger C. Haut, Ph.D.,
Orthopaedic Biomechanics Laboratory,
College
of Osteopathic Medicine,
A414 East
Fee Hall,
Michigan
State University,
East Lansing,
MI 48824,
Tel: (517)355-0320,
Fax: (517)353-0789,
E-mail:
haut@msu.edu